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Custom Rabbit Antibody Production

Abcore is a Southern California antibody production company focused on making high quality antibodies. We emphasize animal health, and our rabbits are bred and put into production on the same farm. This relieves the stress associated with transportation and adjustment to a new environment which happens in a traditional production environment. Abcore also has on site veterinarians which monitor rabbit health and comfort. Healthy rabbits have a more robust immune system, and can produce cleaner, better performing antibodies.

Abcore offers standard 60 day rabbit production packages which consist of 4 immunizations, and 3 production bleeds (please see example project timeline below). However, we can adjust our protocol if you have specific needs. We typically use 2 New Zealand White Rabbits for each project, and prices start at $725.

$725.00 USD Starting Price

1-888-359-1364

orders@abcore-inc.com

1-888-884-9241

Pricing

Features

Package 1

Customer Antigen to Antiserum

Package 2

Customer Antigen to Protein A/G Purified Antibody

Package 3

Customer Antigen to Affinity Purified Antibody

Package 4

Peptide Synthesis to Antiserum

Package 5

Peptide Synthesis to Protein A/G Purified Antibody

Package 6

Peptide Synthesis to Affinity Purified Antibody

Package 7

Affinity Purified Phospho-specific Antibody

service includes

Prebleed

Immunization of 2 rabbits

ELISA titre

Customer provided antigen

Protein A/G purification

Affinity purification

Antigen design

Peptide antigen synthesis

Time (weeks)

8-10 10-12 10-13 12-13 13-15 12-13 13-16

what you recieve

Full project report

ELISA data

Preimmune serum (5mL)

Rabbit antiserum (125mL)

Protein A/G purified antibody (25mL)

Affinity purified antibody (25mL)

Affinity resin (10mL)

Peptide (2-3mg)

what you recieve

Price

$725

$975

$1,225

$1,125

$1,375

$1,695

$3,250

Additional items if Project is Extended Past 56 Days

Per diem board and care

Additional antigen needed

$2.00/Day/Animal (includes egg collection)

$10.00/ml

Schedule

Week Action
Pre-immunization bleed only upon request

0

Immunize rabbits with 0.25 mg of antigen mixed with Complete Freund's Adjuvant

2

Immunize rabbits with 0.2 mg of antigen mixed with Incomplete Freund's Adjuvant

4

Immunize rabbits with 0.2 mg of antigen mixed with Incomplete Freund's Adjuvant

5

Production bleed yielding approximately 20-25 ml rabbit serum

6

Immunize rabbits with 0.2 mg of antigen mixed with Incomplete Freund's Adjuvant

7

Production bleed yielding approximately 20-25 ml rabbit serum

8

Production bleed yielding approximately 20-25 ml rabbit serum
ELISA, western blot or other testing as requested by client
Shipment or Purification of Serum

Process Details

Antigen requirement

Average yield

lgY yield

Amount

Volume

Notes

1.4 mg per 2 chicken protocol for immunizations

12-15 eggs per chicken

Approximately 50 mg/yolk

0.05 mg

0.1 ml

Yolk lgY isolation is available for nominal cost, and can be shipped in PBS containing 0.1% NaN3

We can also ship intact eggs