Conjugated antibodies are antibodies that have been joined or conjugated to a type of second molecule. The second molecule can be a toxin, radioisotope, or other label. Abcore-Inc. offers numerous conjugated antibodies that come labeled with a fluorescent dye and proteins. Conjugated antibodies are also called immonoconjugates and are mostly used in immunotherapy or in development of antibodies used to target specific tissues. There are a few, more newly developed, combination conjugates which include PerCP-Cy5.5, PE-Cy™5, PE-Dyomics 647, PE-Dyomics 590. PE-Dyomics 647 is a dual conjugate that can be attached to antibodies. Phycoerythrin, often abbreviated PE, is a fluorescent dye. PE is comparatively larger than many other types of conjugates and has a molecular weight of 240 kd. The molecule itself contains 25 fluors. Dyomics 647 absorbs and emits light at 653 nm and 672 nm respectively (in Ethanol). Dy-647 is a pentamethine based bluorophore that is soluble in water, methanol, DMF, and DMSO. It produces a red excitation by way of laser. Dy-647 is a pentamethine based fluorophore. The PE-Dyomics 647 combination has an excitation wavelength of 488nm and an emission wavelength of 672 nm.

Anti-CD10 (PE-Dyomics 647) antibody binds against CALLAS (common acute lymphoblastic leukemia antigen). Common acute lymphoblastic leukemia antigen is a characterization of leukemias and lymphomas in blood. CALLA has a thermolysin-like specificity but prefers to act against polypeptides of a particular size (up to 30 amino acids in length). CD10 is located on the cell membrane and is part of the peptidase M13 family. Anti-CD45 (PE-Dyomics 647) antibody (protein tyrosine phosphatase receptor type C antibody) binds against the target CD45 antigen. CD45 (leukocyte common antigen) is a protein tyrosine-protein phosphatase required to activate T-cells. CD45 is a positive regulator of T-cell coactivation that is localized in the membrane of cells. Once the T-cell has been activated by the CD45 activity, SKAP1 and FYN are recruited and dephosphorylated. Defects in protein tyrosine phosphatase receptor type C cause severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell positive and increase one’s susceptibility to multiple sclerosis. Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell positive is a rare disease that presents with a fever, rash, hepatosplenomegal, lymphadenopathy, pneumonitis, and pancytopenia. It is a severe combined immunodeficiency. Multiple sclerosis is an inflammatory autoimmune disease characterized by plaques on the nerve fibers in the brain, spinal cord, and optic nerves.