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Normal Serums

Normal serums are most commonly used for blocking to reduce background staining in immunohistochemistry. Blocking with normal serum from the same species that your secondary antibody was raised in can significantly reduce background when doing immunohistochemistry, immunocytochemistry, or any immunoassay. For example, if your primary antibody was raised in rabbit, then you might use a goat anti-rabbit secondary antibody. In this case, you would use normal goat serum in the first blocking step, before application of your primary antibody. This helps bind up any nonspecific or reactive binding sites. This first incubation with the normal serum should be performed from one hour to overnight, at room temperature to 4 degrees. After this, the primary antibody should be applied. You never want to block with a blocking serum which is the same species that your primary antibody was raised in as this would actually increase background. In our above example, the primary antibody is raised in rabbit, so you would never want to use normal rabbit serum. If you used normal rabbit serum, immunoglobulins present in the normal serum would bind to nonspecific reactive sites, which would then bind your secondary antibody and create nonspecific background signal. If used correctly, blocking with normal serums can increase the sensitivity of your immunoassay, as well as improving the signal to noise ratio.

 
Featured Products
Product Number Title Applications Host Clonality
AC15-0101 Normal Mouse Serum Blocking
AC15-0113 Normal Cat Serum Blocking
AC15-0112 Normal Pig Serum Blocking
AC15-0111 Normal Donkey Serum Blocking
AC15-0110 Normal Horse Serum Blocking
AC15-0109 Normal Syrian Hamster Serum Blocking
AC15-0108 Normal Guinea Pig Serum Blocking
AC15-0106 Normal Bovine Serum Blocking
AC15-0105 Normal Rat Serum Blocking
AC15-0104 Normal Sheep Serum Blocking
AC15-0100 Normal Human Serum Blocking
AC15-0115 Normal Llama Serum Blocking
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