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Insect Cell Media

baculoGROW™ Insect Cell Media

baculoGROW™ is a serum- and protein-free medium. The insect cell media has been specially formulated to facilitate the growth of Spodoptera frugiperda cell lines (Such as Sf9 cells and Sf21 cells), Drosophila S2 cells and other cells such as Trichoplusia ni (T.ni or Hi-5) cells. baculoGROW™ can be used for culturing adherent cells and suspension cultures, such as those used in bioreactors.

Key Features

  • Ideally suited to use with the baculovirus expression system
  • Protein free, fully defined, complete medium
  • Supports cell densities higher than 10E7 cells/ml
  • Supports high recombinant protein yields
  • Contains Pluronic F68 for minimized shear forces
  • Can be used as a freezing medium
  • No adaptation from cultures containing serum is needed

Storage and Shelf Life

  • Store at 2-8°C. baculoGROW™ insect cell media is light sensitive so should be protected from light during storage.
  • baculoGROW™ insect cell media has a shelf life of 9 months.

General guidelines for insect cell culture

Insect cells should be cultured at 27 – 28 °C in a non-humidified atmosphere, without CO2. Insect cell cultures may be grown in a variety of vessels, such as shake flasks (100 – 140 rpm), spinner flasks or WAVE bags. The caps on all insect cell culture flasks should be loosened and adequate vessel headspace should be given to provide gas exchange.

For more information, please download the baculoGROW™ Information Sheet (PDF).

baculoGROW™ insect cell media is also included as an integral part of the new Baculovirus Expression System, baculoCOMPLETE™.

 
Product Number Title Applications Host Clonality
500100 baculoGROW