Questions? Feedback? powered by Olark live chat software

Hedgehog Signaling

Stem cells are undifferentiated cells that have the capability to differentiate into specialized cell types. Stem cells are found in embryos during the blastocyte phase and also in adult tissue. A blastocyte is a 4 or 5 day old embryo. These stem cells can be harvested from embryos produced during the in-vitro fertilization process which may not be implanted or used in assisted reproduction attempts. There are many stem cell signaling pathways that can determine the fate of a stem cell. These pathways are only somewhat understood and include the notch pathway, wingless-type pathway (Wnt), smad pathway, and sonic hedgehog pathway (Shh). . The hedgehog pathway activates a certain ligand called "smoothened" by way of deactivating a protein called "patched". This essentially triggers a signaling cascade that results in the transcription of certain genes that are involved in cell proliferation, development, and tissue maintenance. The hedgehog pathway is highly regulated by checkpoints. The hedgehog gene itself encodes for the secreted protein PTCH. PTCH is a transmembrane receptor that acts as a ligand binding to smoothened. When patched is bound to smoothened, it is essentially in a resting and deactivated state. When hedgehog binds to the PTCH receptor, it is degraded and "smoothened" is made available to continue on the signaling cascade. Smoothened activates the gli family of transcription factors and initiates the transcription of many different target genes.

Anti-Sonic Hedgehog antibody, anti-SIAH1, binds against SIAH1 target. The target binds to a patched receptor which functions with other receptors to activate the transcription of target genes. Sonic Hedgehog protein is expressed in the fetal intestine, fetal liver, fetal lung, and fetal kidney. It is not expressed in adult tissues. Hedgehog family proteins help cells in a growing embryo develop into different body parts. The Sonic Hedgehog pathway plays a key role in regulating organogenesis in vertebrates. Defects in SIAH1 cause a number of disorders like basal cell carcinoma, microphthalmia isolated with coloboma type 5, holoprosencephaly type 3, and solitary median maxillary central incisor syndrome. Basal cell carcinoma is a slow growing form of nonmelanoma skin cancer. Microphthalmia isolated with coloboma type 5 is a rare inherited disorder characterized by small eyes and eye colobomas. Solitary median maxillary central incisor is a very rare syndrome characterized by various midline defects of development, including a single centered incisor on the top jaw (maxilla) only. Anti-smoothened homolog antibody binds against the target protein smoothened homolog. Smoothened homolog is localized on the cell membrane. It is a G-protein coupled receptor that associates with the patched protein to transduce the hedgehog's proteins signal.

 
Product Number Title Applications Host Clonality
AC21-2842 Anti-PTCHD3 Antibody ELISA, WB Goat Polyclonal
AC21-2848 Anti-Patched / PTCH Antibody ELISA, WB Goat Polyclonal
AC21-0823-01 Anti-Patched / PTCH Antibody (AMCA) ELISA, IHC Goat Polyclonal
AC21-0823-02 Anti-Patched / PTCH Antibody (AP) ELISA, IHC Goat Polyclonal
AC21-0823-03 Anti-Patched / PTCH Antibody (APC) ELISA, IHC Goat Polyclonal
AC21-0823-04 Anti-Patched / PTCH Antibody (APC-Cy5.5) ELISA, IHC Goat Polyclonal
AC21-0823-05 Anti-Patched / PTCH Antibody (APC-Cy7) ELISA, IHC Goat Polyclonal
AC21-0823-06 Anti-Patched / PTCH Antibody (Avidin) ELISA, IHC Goat Polyclonal
AC21-0823-07 Anti-Patched / PTCH Antibody (Biotin) ELISA, IHC Goat Polyclonal
AC21-0823-08 Anti-Patched / PTCH Antibody (BPE) ELISA, IHC Goat Polyclonal
AC21-0823-09 Anti-Patched / PTCH Antibody (Cy3) ELISA, IHC Goat Polyclonal
AC21-0823-10 Anti-Patched / PTCH Antibody (Cy5) ELISA, IHC Goat Polyclonal