Custom Goat Antibody Production
Abcore produces quality custom goat polyclonal antibodies due to the great care we give our animals. Our goats are bred in the same area as our farm in Southern California. They are designated as disease-free by the local USDA office. The expert veterinary care and daily health assessment insures that our clients are receiving the best chance for target antibody development. We typically use female yearlings for each project, and prices start at $1,320.
Abcore provides free antigen design to assist in selection of the best possible peptide sequence for eliciting the desired antibody response. We use the latest in software with the proper algorithms to assess antigenicity, hydrophilicity, flexibility, secondary structure and aggregation potential. Please take advantage of this free assistance.
Pricing
| Features |
Package 1 Customer Antigen to Antiserum |
Package 2 Customer Antigen to Protein A/G Purified Antibody |
Package 3 Customer Antigen to Affinity Purified Antibody |
Package 4 Peptide Synthesis to Antiserum |
Package 5 Peptide Synthesis to Protein A/G Purified Antibody |
Package 6 Peptide Synthesis to Affinity Purified Antibody |
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service includes |
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Prebleed |
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Immunization of 1 goat |
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ELISA titre |
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Customer provided antigen |
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Protein A/G purification |
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Affinity purification |
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Antigen design |
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Peptide antigen synthesis |
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Time (weeks) |
14-16 | 16-18 | 16-18 | 16-18 | 16-18 | 17-19 |
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what you recieve |
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Full project report |
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ELISA data |
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Preimmune serum (5mL) |
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Rabbit antiserum (750mL) |
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Protein A/G purified antibody (25mL) |
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Affinity purified antibody (25mL) |
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Affinity resin (10mL) |
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Peptide (2-3mg) |
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Pricing |
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Price |
$1,320 |
$1,520 |
$1,780 |
$1,700 |
$1,820 |
$2,150 |
Additional items if Project is Extended Past 56 Days
Per diem board and care
Additional antigen needed
$2.00/Day/Animal (includes egg collection)
$10.00/ml
Schedule
| Week | Action |
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| Pre-immunization bleed only upon request | |
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0 |
Immunize goat with 0.5 mg of antigen mixed with Complete Freund's Adjuvant |
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2 |
Immunize goat with 0.5 mg of antigen mixed with Incomplete Freund's Adjuvant |
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4 |
Immunize goat with 0.5 mg of antigen mixed with Incomplete Freund's Adjuvant |
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6 |
Production bleed yielding approximately 250-275 ml goat serum |
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8 |
Immunize goat with 0.5 mg of antigen mixed with Incomplete Freund's Adjuvant |
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10 |
Production bleed yielding approximately 250-275 ml goat serum |
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12 |
Immunize goat with 0.5 mg of antigen mixed with Incomplete Freund's Adjuvant |
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14 |
Production bleed yielding approximately 250-275 ml goat serum |
Process Details
Antigen requirement
Average yield
lgY yield
Amount
Volume
Notes
1.4 mg per 2 chicken protocol for immunizations
12-15 eggs per chicken
Approximately 50 mg/yolk
0.05 mg
0.1 ml
Yolk lgY isolation is available for nominal cost, and can be shipped in PBS containing 0.1% NaN3
We can also ship intact eggs