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G Protein Coupled Receptors

G-protein antibodies are useful in many areas of research. G-proteins are a group of proteins that have a unique mechanism of action used to transmit chemical signals. Simplified, the process involves a binding of a ligand on the surface of a cell to a G protein coupled receptor. This transmits a signal into the cell which activates a G protein. This protein undergoes certain changes and is essentially activated, released by the receptor, and able to bind to distant binding sites. More expanded, a ligand binds to a receptor outside of the cell wall. The end of the receptor on the inside of the cell wall takes on certain conformational changes. It moves and changes shape effectively turning into a docking site for a G-protein. The G-protein will bind to the docking site and then undergo certain conformational changes within it. G-proteins have 3 subunits labeled alpha, beta, and gamma. Inactivated G-proteins carry GDP molecules or GTP molecules on their alpha subunits. When GDP is attached, the G protein remains a whole piece comprised of its 3 subunits. When the G-protein is activated it releases the GDP molecule and exchanges it for a GTP molecule. This process is initiated by the binding of the external ligand and effectively dissociates the alpha subunit from the beta-gamma complex. The GTP-alpha complex is then free (with the assistance of the enzyme adenylate cyclase) to move on and bind to other receptor cites at different locations. The unique action of the G-protein allows it to indirectly control ion channels without directly binding to them. The 5HT3B receptor is one of several receptors for the neurochemical serotonin (5-hydroxytryptamine). Serotonin is a neurotransmitter and a hormone responsible for the regulation of satiety, hunger, mood, and sleep.

Anti- 5HT3B antibody binds to this receptor in place of serotonin and can effectively block the binding of 5HT3B. The receptor itself is cation exclusive but otherwise is a comparatively non-selective ion channel. Defects in serotonin receptors can lead to disorders like depression, obesity, autism, and problems with sleep. The anti-Von Hippel Lindau antibody has involvement in cancer, metabolism, transcription, and cell-cycle functions. Also known as the VHL antibody, it can bind to the von Hippel-Lindau ugiquitination complex. The target protein is involved in the ubiquitination and proteasomal degradation of the VHL complex. Defects in this complex cause von Hippel-Lindau disease, pheochromocytoma, and erythrocytosis familial type 2. VHL is a rare genetic disorder in which affected individuals develop abnormal tumor growth in multiple parts of the body. Pheochromocytoma is a rare tumor of the adrenal gland which results in excess epinephrine and norepinephrine transmission. It is characterized by irregular heart rate, upset metabolism, and blood pressure. Erythrocytosis familial type 2 is an autosomal recessive genetic disorder which causes red blood cells to increase in mass. Complications of this disease include peripheral thrombosis and cerebrovascular events.

 
Product Number Title Applications Host Clonality
AC16-0042 Anti-EDG1 Antibody WB, IHC(F), IHC(P) Mouse Monoclonal (2B9)
AC16-0042-01 Anti-EDG1 Antibody (AMCA) WB, IHC(F), IHC(P) Mouse Monoclonal (2B9)
AC16-0042-02 Anti-EDG1 Antibody (AP) WB, IHC(F), IHC(P) Mouse Monoclonal (2B9)
AC16-0042-03 Anti-EDG1 Antibody (APC) WB, IHC(F), IHC(P) Mouse Monoclonal (2B9)
AC16-0042-04 Anti-EDG1 Antibody (APC-Cy5.5) WB, IHC(F), IHC(P) Mouse Monoclonal (2B9)
AC16-0042-05 Anti-EDG1 Antibody (APC-Cy7) WB, IHC(F), IHC(P) Mouse Monoclonal (2B9)
AC16-0042-06 Anti-EDG1 Antibody (Avidin) WB, IHC(F), IHC(P) Mouse Monoclonal (2B9)
AC16-0042-07 Anti-EDG1 Antibody (Biotin) WB, IHC(F), IHC(P) Mouse Monoclonal (2B9)
AC16-0042-08 Anti-EDG1 Antibody (BPE) WB, IHC(F), IHC(P) Mouse Monoclonal (2B9)
AC16-0042-09 Anti-EDG1 Antibody (Cy3) WB, IHC(F), IHC(P) Mouse Monoclonal (2B9)
AC16-0042-10 Anti-EDG1 Antibody (Cy5) WB, IHC(F), IHC(P) Mouse Monoclonal (2B9)
AC16-0042-11 Anti-EDG1 Antibody (Cy5.5) WB, IHC(F), IHC(P) Mouse Monoclonal (2B9)