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flashBACGOLD

flashBACGOLD™ is a further improvement of the flashBAC™ system, by the removal of both chiA and a protease (v-cath) from the virus genome. These modifications are primarily designed to facilitate the movement of recombinant proteins through the cell's secretory pathway and to prevent their degradation once they are released into the culture media.

Complex secretory or membrane-bound glycoproteins are often more difficult to express using baculovirus and produced in lower amounts compared to cytoplasmic or nuclear proteins 1,2,3,4,5.

flashBACGOLD™ is a baculovirus expression vector that has been designed to reduce proteolysis, maximise protein secretion and improve membrane protein targeting and is based on our patented flashBAC™ system, removing the necessity for plaque-purification.

As such, it is also back-compatible with all existing baculovirus transfer vectors based on homologous recombination in insect cells at the polyhedrin locus.

Baculovirus DNA chiA
deleted
v-cath
deleted
P10/P26/P74
deleted
Applications
flashBAC™ • Expression of secreted and membrane targeted proteins
flashBACGOLD™ • Reduced proteolysis
• Improved secretion and membrane targeting
flashBACULTRA™ • Increased transcription of polh promoter giving higher yields
• Increased protein quality due to improved cellular stability
• Reduced proteolysis
• Improved secretion and membrane targeting

For a listing of recent publications which have used the flashBAC expression system, please see our listing of flashBAC publications.

For more information and protocol details, please download the flashBAC™ Userguide (PDF).

flashBACGOLD™ Certificate of Analysis (PDF)

 
Product Number Title Applications Host Clonality
100200 flashBACGOLD
100201 flashBACGOLD
100202 flashBACGOLD
100203 flashBACGOLD