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Embryonic Stem Cells

Embryonic stem cells are undifferentiated cells that are harvested from embryos. These cells have the capability to differentiate into a slew of different types of cells and furthermore tissues and organs. Typically embryonic stem cells are harvested from embryos that have been fertilized in vitro, not inside of a woman's body. Many of these embryos are left over at fertility clinics from successful attempts at in vitro fertilization. Typically, not all of the embryos are implanted into a prospective mother and turn into viable pregnancies. Some people decide to donate these to research, from which embryonic stem cells can be harvested. After the stem cells are harvested, they are grown in the laboratory as a cell culture. The culture is grown in a petri dish over specialized feeder layer of cells in a nutrient rich culture medium. The process of growing stem cells is extremely inefficient and not all of the cells will survive and divide. However, those that do survive and divide are continually separated and allowed to divide and continue to grow in culture. These cells that continue to multiply but not differentiate or develop abnormalities are considered pluripotent and become embryonic stem cell lines. These embryonic stem cell lines can be differentiated into different types of cells or even injected into immunodefficient mice to see if tissues develop. Typically successful embryonic stem cell lines, when injected into immunodeficient mice, will develop teratomas. Teratomas are tumors which include many different types of tissues, both partially differentiated and fully developed.

Anti-Oct4 antibody (Anti-POU5F1 antibody) binds against the target Oct4. Oct4 is a transcription factor that binds to the octamer motif and forms a trimeric complex with SOX2 on DNA. This complex controls the expression of a number of genes involved in embryonic development. POU5F1 is expressed in the developing brain and localized in the nucleus. Anti-nanog antibody binds against the target nanog. Nanog is localized in the cell nucleus and expressed in testicular cancer tumors, fetal gonads, and ovary cancer cells. Nanog is a transcription factor regulator involved in inner cell mass and embryonic stem cell proliferation and self-renewal.

 
Product Number Title Applications Host Clonality
AC16-0036 Anti-SOX17 Antibody ELISA, WB, IHC(F), IHC(P), IP Mouse Monoclonal (BC24-3.5CH)
AC16-0045 Anti-KLF4 Antibody ELISA, WB Rabbit Polyclonal
AC16-0046 Anti-Oct4 Antibody WB Rabbit Polyclonal
AC16-0036-01 Anti-SOX17 Antibody (AMCA) ELISA, WB, IHC(F), IHC(P), IP Mouse Monoclonal (BC24-3.5CH)
AC16-0036-02 Anti-SOX17 Antibody (AP) ELISA, WB, IHC(F), IHC(P), IP Mouse Monoclonal (BC24-3.5CH)
AC16-0036-03 Anti-SOX17 Antibody (APC) ELISA, WB, IHC(F), IHC(P), IP Mouse Monoclonal (BC24-3.5CH)
AC16-0036-04 Anti-SOX17 Antibody (APC-Cy5.5) ELISA, WB, IHC(F), IHC(P), IP Mouse Monoclonal (BC24-3.5CH)
AC16-0036-05 Anti-SOX17 Antibody (APC-Cy7) ELISA, WB, IHC(F), IHC(P), IP Mouse Monoclonal (BC24-3.5CH)
AC16-0036-06 Anti-SOX17 Antibody (Avidin) ELISA, WB, IHC(F), IHC(P), IP Mouse Monoclonal (BC24-3.5CH)
AC16-0036-07 Anti-SOX17 Antibody (Biotin) ELISA, WB, IHC(F), IHC(P), IP Mouse Monoclonal (BC24-3.5CH)
AC16-0036-08 Anti-SOX17 Antibody (BPE) ELISA, WB, IHC(F), IHC(P), IP Mouse Monoclonal (BC24-3.5CH)
AC16-0036-09 Anti-SOX17 Antibody (Cy3) ELISA, WB, IHC(F), IHC(P), IP Mouse Monoclonal (BC24-3.5CH)