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Baculovirus Titration Kit

baculoQUANT all-in-one

A convenient "all reagents included" virus extraction and quantification kit

No more plaque assays, no more ELISA or immuno assays, and no more virus extraction spin columns!

Key Features:

  • Titer accuracy comparable to plaque assay method
  • Less than one hour hands-on time
  • Virus DNA extraction and titration in under three hours
  • Compatible with any AcMNPV-based baculovirus system containing gp64 viral gene
  • Single step virus DNA extraction: just add lysis solution (no spin columns)
  • QPCR Primers/ Probe provided as a single reagent mix: just add water
  • High performance, ultra sensitive master mix reagent included
  • Plaque assay titred virus internal standards included for standard curve generation

Overview of the Protocol

  1. Virus DNA is extracted from recombinant viruses of unknown titer and from a series of dilutions of the virus internal standard (no columns are needed, simply add lysis solution to the virus sample and place into a thermocycler).
  2. QPCR is carried out to determine the Ct values for the virus DNA samples and internal standards (realtime PCR machine).
  3. The titres of the virus DNA samples are determined by plotting their Ct values onto a standard curve generated using virus internal standards (provided in the kit).

Kit Contents:

  • Lysis buffer
  • Primer/Probe mix
  • QPCR master mix
  • Virus internal standard
  • DNA positive control
  • RNase free water
  • User Guide

The kit contains reagents adequate for 100 reactions. This allows for up to 24 titrations in triplicate and 3 standard curves.

For more information and protocol details, please download the baculoQUANT all-in-one User Guide (PDF).

Please note this product ships on dry ice so additional shipping charges may apply.

 
Product Number Title Applications Host Clonality
100602 baculoQUANT all-in-one