Baculovirus DNA	chiA deleted	v-cath deleted	P10/P26/P74 deleted	Applications
flashBAC™				• Expression of secreted and membrane targeted proteins
flashBACGOLD™				• Reduced proteolysis • Improved secretion and membrane targeting
flashBACULTRA™				• Increased transcription of polh promoter giving higher yields • Increased protein quality due to improved cellular stability • Reduced proteolysis • Improved secretion and membrane targeting

flashBAC™ is an expression system that allows the production of multiple recombinant viruses in a one-step process [1]. This technology has greatly de-skilled the process of making recombinant viruses and increased the throughput of recombinant protein production. It also has a chitinase gene deletion (chiA) that greatly improves the movement of recombinant proteins through the cellular secretory pathway.

flashBACGOLD™ is a further improvement on this system, by the removal of both chiA and a protease (v-cath) from the virus genome [2]. These modifications are primarily designed to facilitate the movement of recombinant proteins through the cell's secretory pathway and to prevent their degradation once they are released into the culture media.

flashBACULTRA™ has taken this technology a step further, by the removal of three more virus genes (p10, p74 and p26) from the flashBACULTRATM genome.

flashBACPRIME™ was designed with virus like particle production in mind, combining the simplicity and easy to use flashBAC one step baculovirus expression system with increased recovery and yield of virus like particles.

For more information and protocol details, please download the flashBACULTRA™ Userguide (PDF).