Questions? Feedback? powered by Olark live chat software

Antibody purification is performed to concentrate and enrich antigen-specific antibodies and lower background by removing any non-specific proteins. This step is performed when highly purified antibodies are required, such as in immunohistochemistry and immunocytochemistry.

Abcore can purify your monoclonal antibodies and polyclonal antibodies, utilizing a wide array of protein purification methods. We can purify milligram quantities of antibody for research purposes, all the way up to multi-gram quantities. We can purify ascites fluid, animal serum, and tissue culture supernatant. Our goal is to find or develop the antibody purification procedure which provides the best balance between yield, purity, and cost. Available antibody purification services and methods include:

Antigen Affinity Purification

Antigen affinity chromatography selectively purifies the specific antibody of interest from the remaining antibodies in the antiserum. Antibodies of extremely high purity and specificity can be obtained using custom affinity chromatography. A minimum of 10 mg of pure antigen (or purified peptide) is needed to perform this procedure. It's mainly used with polyclonal antiserum in which specific antibody comprises only 5-10% of the total immunoglobulin.

Protein A and Protein G Affinity Chromatography

Protein A Purifications

The protein A purification process binds the Fc region of major sub-classes of mouse and rat IgG antibodies to varying degrees. The results with protein A purifications are usually high purity antibody and high yields. It is useful for large-scale purifications of tissue culture supernatants.

Protein G Purifications

Protein G can be used for purifying antibodies from most sources. Like protein A, the result of protein G antibody purificaion are usually of high purity and high yield. It is applicable to a wider range of immunoglobulin’s than protein A, but elution procedures can be much harsher.

Ammonium Sulfate Precipitation

Ammonium sulfate precipitation is useful for concentration and partial purification of antibodies from most sources and all species. Although, on its own the yields of antibody are impure it provides crude purification of total immunoglobulin from other serum proteins. It is widely used in isolating both IgM antibodies and IgG antibodies from a variety of species. A gel filtration and/or an ion exchange step can be employed to further purify the immunoglobulin of interest.

PEG Purification

PEG Purification is used to purify mouse IgM and mouse IgG3 antibodies since they will not bind to protein A or protein G.

For more information, please download the antibody purification order form (PDF).